Coding

Part:BBa_K4205005:Design

Designed by: Yuq iHuang   Group: iGEM22_NJMU-China   (2022-09-10)


AcmA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 703
    Illegal SpeI site found at 1192
    Illegal SpeI site found at 1216
    Illegal SpeI site found at 1237
    Illegal SpeI site found at 1258
    Illegal SpeI site found at 2167
    Illegal SpeI site found at 2203
    Illegal PstI site found at 69
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2353
    Illegal SpeI site found at 703
    Illegal SpeI site found at 1192
    Illegal SpeI site found at 1216
    Illegal SpeI site found at 1237
    Illegal SpeI site found at 1258
    Illegal SpeI site found at 2167
    Illegal SpeI site found at 2203
    Illegal PstI site found at 69
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 703
    Illegal SpeI site found at 1192
    Illegal SpeI site found at 1216
    Illegal SpeI site found at 1237
    Illegal SpeI site found at 1258
    Illegal SpeI site found at 2167
    Illegal SpeI site found at 2203
    Illegal PstI site found at 69
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 703
    Illegal SpeI site found at 1192
    Illegal SpeI site found at 1216
    Illegal SpeI site found at 1237
    Illegal SpeI site found at 1258
    Illegal SpeI site found at 2167
    Illegal SpeI site found at 2203
    Illegal PstI site found at 69
    Illegal AgeI site found at 1336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers: forward - 5'-GCTACCTGCTTAGCCAATCTTAGCAACGTAAT-3' reverse - 5'-CATGCATGCATGAATTTTAAATCACAAAATCCAA-3' to PCR AcmA from the genome of Lactobacillus plantarum-168.


Source

Obtained by PCR on the genome of Lactobacillus plantarum-168.

References